ABSTRACT
Background: Streptococcus mutans is one of the most important cariogenic species of the human oral microbial flora. Biofilm style of microbial growth thought to resist the actions of antimicrobials. Aim: The purpose of this study was to evaluate the antimicrobial efficacy of Triphala, and 0.2% chlorhexidine against S. mutans biofilm formed on tooth substrate. Settings and Design: Randomized control trial. Methods: Extracted human mandibular premolars sectioned below the cemento‑enamel junction were placed in the tissue culture wells exposing the crown surface to S. mutans to form a biofilm. At the end of 3rd and 7th day, all groups were treated for 10 min with the test solutions and control and were analyzed qualitatively and quantitatively. Statistical Analysis Used: One‑way ANOVA. Results: Qualitative assay with 3 days biofilm showed complete inhibition of bacterial growth with Triphala, but 0.2% chlorhexidine and saline showed the presence of bacterial growth. In quantitative analysis, 0.2% chlorhexidine and Saline treated tooth samples have shown 1052 × 104 ± 15.1 × 104 CFU/ml, 141.3 × 109 ± 2.1×109 CFU/ml, respectively. Qualitative assay with 7 days biofilm on crown portion showed dense growth when treated with 0.2% chlorhexidine and saline, whereas Triphala has shown minimal growth. In Quantitative analysis, Triphala showed statistically significant result when compared with 0.2% chlorhexidine and saline. Conclusion: Triphala showed statistically significant antibacterial activity against S. mutans biofilm formed on tooth substrate. The incorporation of Triphala in mouth rinse could prove to be effective in reducing S. mutans count in the oral cavity.
Subject(s)
Anti-Infective Agents/therapeutic use , Biofilms , /therapeutic use , Dental Enamel , India , Mouthwashes/therapeutic use , Plant Extracts/therapeutic useABSTRACT
The aim of this study was to radiographically investigate the curve existing in slightly curved mesiobuccal canal of mandibular first molar by applying Piecewise straight line approximation. Extracted human mandibular molars were radiographed and one hundred radiographs were selected whose mesiobuccal canal showed slight curvature (10-20°) according to Schneider's method. The curves were traced and analyzed using Piecewise straight line method. Each curve was considered as a unit consisting of six different pieces of straight lines joining at seven specific points and the angle of curvature at these points was determined using the slope formula. All curves analyzed in this study had varying degrees of curvatures at different points on the curve. Maximum curvature (0.40°) was recorded at the middle third of the root canal. Within the limitation of the study, significant curvature occurs through out the curve existing in the mesiobuccal canal of mandibular first molar and the middle third of the curve showing greater degree of curvature. There is a possibility of greater curvature occurring in the coronal third of the curve. Though three dimensional studies would be more appropriate, Piecewise straight line approximation may be a better method than existing methods to simulate canal geometry.
El objetivo de este estudio fue investigar radiográficamente la curva existente en el canal radicular mesiobucal ligeramente curvado del primer molar inferior mediante la aplicación de aproximaciones a segmentos de línea recta. Se utilizaron molares inferiores humanos extraídos que fueron radiografiados. Fueron seleccionadas, según el método de Schneider, 100 radiografías cuyo canal mesiobucal mostró una ligera curvatura (10-20°). Las curvas fueron delineadas y analizadas mediante el método de aproximación a segmentos de línea recta. Cada curva se consideró como una unidad que consta de seis diferentes segmentos de línea recta que unen a siete puntos específicos y el ángulo de curvatura en estos puntos se determinó utilizando la fórmula de la pendiente. Todas las curvas analizadas en este estudio tenían diversos grados de curvaturas en diferentes puntos de la curva. La curvatura máxima (0,40°) se registró en el tercio medio del canal radicular. Dentro de la limitación del estudio, una curvatura significativa se produce a través de la curva existente en el canal mesiobucal del primer molar inferior y el tercio medio de la curva que muestra un mayor grado de curvatura. Existe la posibilidad que ocurra una mayor curvatura en el tercio coronal de la curva. Aunque un estudio tridimensional sería más apropiado, la aproximación a segmentos de línea recta puede ser un mejor método que los existentes para simular la geometría del canal.